A biological method of assay of hyaluronidase
نویسندگان
چکیده
منابع مشابه
Hyaluronidase: correlation between biological assay and other methods of assay.
Different workers have at various times sought to correlate the in vivo and in vitro activities of hyaluronidase preparations. Chain & Duthie (1940), drawing attention to the association between diffusion in the tissues and the mucolytic activity of testicular extracts, pointed out that the viscosityreducing and diffusing activities of various enzyme preparations were in good agreement. McClean...
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A variety of methods have been devised for the assay of the enzyme hyaluronidase. These may be divided into four types: (a) measurement of the “spreading effect” in the skin of rabbits and guinea pigs (1, 2), (b) reduction of viscosity (3,4), (c) prevention of mucin clot formation (5), and (d) chemical measurement of total reducing substances, N-acetylglucosamine, or glucuronic acid liberated o...
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(1) The spreading reaction is a biological method, measuring the decomposing effect of hyaluronidase on the mucoids of the cement substance of rabbit's skin (Duran-Reynals, 1929). These substances consist partly of hyaluronic acid. Hyaluronidase increases the permeability of the tissues, and this involves an increased spreading capacity of liquids and corpuscular substances. This test is not sp...
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(1) The spreading reaction is a biological method, measuring the decomposing effect of hyaluronidase on the mucoids of the cement substance of rabbit's skin (Duran-Reynals, 1929). These substances consist partly of hyaluronic acid. Hyaluronidase increases the permeability of the tissues, and this involves an increased spreading capacity of liquids and corpuscular substances. This test is not sp...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1953
ISSN: 0306-3283
DOI: 10.1042/bj0530056